immunoregulatory cytokine (tgf-ß and il-10) responses in mice inoculated with protoscoleces and major hydatid fluid antigens of cystic echinococcosis
نویسندگان
چکیده
background: our objectives were to investigate whether immunomodulatory cytokines, tgf-β and il-10, are stimulated in response to cystic echinococcosis (ce) components in mice model, and whether major hydatid fluid antigens or live protoscoleces could equally contribute to such cytokines. methods: protoscoleces were obtained by aseptic puncture of fertile sheep hydatid cysts. hydatid fluid antigens (hfags) and antigen b (agb) were prepared by partial purification and electroelution, respectively. of the 25 balb/c mice assigned in four groups, the first group was inoculated ip with 2000 live protoscoleces; the second and the third groups were injected ip with 50 µg hfags and 50 µg agb in 200 µl of pbs, respectively. control group was only injected with pbs. the sera concentration of tgf-ß and il-10 were determined by elisa. data were analyzed using one-way anova and tukey-hsd tests to compare differences between means. results: the mean concentration of tgf-ß in those groups injected with protoscoleces, hfags and agb were significantly higher than control group. however, in the case of il-10 such differences were only detected in mice that were inoculated with protoscoleces (356±11 pg/ml) compared to control (207±9 pg/ml), hfags and agb groups. conclusion: tgf-ß and il-10, two important immunomudulatory cytokines are induced by different molecules or components of ce, so that agb could induce tgf-ß and components of protoscolex, other than agb and ag5, could induce il-10.
منابع مشابه
Dot-Elisa for evaluation of hydatid cyst wall, protoscoleces and hydatid cyst fluid antigens in the serodiagnosis of cystic echinococcosis.
The aim of the present study is to evaluate cyst wall and protoscolex as an alternate source of antigen in serodiagnosis of cystic echinococcosis (CE). A total of 90 blood samples, 30 each of confirmed CE cases, disease controls and healthy controls were collected. Dot-ELISA using cyst wall, protoscolex and cyst fluid were used to demonstrate anti-hydatid antibodies. The sensitivity of Dot-ELIS...
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متن کاملfaculty of psychology and social sciences group of anthropology master thesis in major of anthropology
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Immunization of Lambs against infection with Echinucoccus granulsus using protoscolices and hydatid fluid antigens
متن کامل
Immune Responses to Antigens of in vitro Reared Echinococcus granulosus Adult Worms in Balb/c Mice
Background: Cystic echinococcosis (CE), also known as echinococcosis/hydatidosis, is one of the most important parasitic diseases in the world. It enhances both humoral and cellular (Th1 and Th2) responses in infected host. Different antigens of the worm may favor the Th1 or Th2 immune responses in CE patients. Objective: To evaluate the humoral and cellular immune responses of Balb/c mice agai...
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عنوان ژورنال:
iranian journal of parasitologyجلد ۳، شماره ۳، صفحات ۱۸-۲۳
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